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Affinity Biosciences active il-1β antibody
Active Il 1β Antibody, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
active il-1β antibody - by Bioz Stars, 2026-10
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Fig. 1. Angiotensin II-induced ROS production is mediated by TRIM6. Transfection of HK2 cells was performed with TRIM6- interfering lentivirus or with control virus for 24 h. The cells were then treated with <t>1</t> µM Ang II for 24 h. (A) TRIM6 protein expression.
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Fig. 1. Angiotensin II-induced ROS production is mediated by TRIM6. Transfection of HK2 cells was performed with TRIM6- interfering lentivirus or with control virus for 24 h. The cells were then treated with 1 µM Ang II for 24 h. (A) TRIM6 protein expression.

Journal: Frontiers in bioscience (Landmark edition)

Article Title: TRIM6 Promotes ROS-Mediated Inflammasome Activation and Pyroptosis in Renal Tubular Epithelial Cells via Ubiquitination and Degradation of GPX3 Protein.

doi: 10.31083/j.fbl2902058

Figure Lengend Snippet: Fig. 1. Angiotensin II-induced ROS production is mediated by TRIM6. Transfection of HK2 cells was performed with TRIM6- interfering lentivirus or with control virus for 24 h. The cells were then treated with 1 µM Ang II for 24 h. (A) TRIM6 protein expression.

Article Snippet: After blocking with 5% skim milk, the membranes were incubated overnight at 4 °C with primary antibodies against active IL-1β (#63124, CST, Danvers, MA, USA), IL-1β (#16806-1- AP, Proteintech, Wuhan, China), Caspase-1 (#Ab179515, Abcam, Cambridge, MA, USA), cleaved Caspase-1 (#89332, CST), TRIM6 (#11953-1-AP, Proteintech), GPX3 (#Ab256470, Abcam), NLRP3 (#Ab263899, Abcam), GSDMD (#AF4013, Affinity Biosciences, Cincinnati, OH, USA), GSDMD-N (#Ab215203, Abcam), or GAPDH (#60004-1-Ig, Proteintech), follow by incubation with a secondary antibody (#A0208, #A0216, Beyotime, Shanghai, China).

Techniques: Transfection, Control, Virus, Expressing